Evaluating Dose- and Time-Dependent Effects of Vitamin C Treatment on a Parkinson’s Disease Fly Model

Figure 5

The effects of vitamin C at 0.5 mM on the number of DA neurons in 15-day-old flies. (a) Immunostaining images of brains from control (TH-Control) and dUCH knockdown flies (TH-dUCH KD) using anti-TH antibody. PPM1/2 and PPM3 clusters are enclosed by white dashed boxes and shown with higher magnifications in lower panels (A₅–A₈ and A₉–A₁₂, respectively). Scale bars indicate 100 µm (A₁–A₄), 20 µm (A₅–A₈), and 10 µm (A₉–A₁₂). (b) Quantification of dopaminergic neurons in control and dUCH knockdown flies at 15 days. One-way ANOVA with Tukey's test, and and , , and error bars indicate the standard deviation of data. TH-Control (+; +; TH-GAL4/+) and TH-dUCH KD (+; +; TH-GAL4/UAS-dUCH.IR).

(a)

(b)