Evaluating Dose- and Time-Dependent Effects of Vitamin C Treatment on a Parkinson’s Disease Fly Model

Figure 6

The effects of vitamin C at 0.5 mM on the number of DA neurons in 30-day-old flies. Immunostaining images of brains from control (TH-Control) and dUCH knockdown flies (TH-dUCH KD) using anti-TH antibody. The whole central brains were shown in A₁–D₁. PAL, PPM1/2, PPM3, PPL1, and PPL2 clusters are enclosed by white dashed boxes and shown with higher magnifications in lower panels (A₂–D₂, A₃–D₃, A₄–D₄, A₅–D₅, and A₆–D₆, respectively). Scale bars indicate 100 µm (A₁–D₁), 20 µm (A₃–D₃), and 10 µm (A₂–D₂, A₄–D₄, A₅–D₅, and A₆–D₆). TH-Control (+; +; TH-GAL4/+) and TH-dUCH KD (+; +; TH-GAL4/UAS-dUCH.IR).