Phosphorylated Alpha-Synuclein in Red Blood Cells as a Potential Diagnostic Biomarker for Multiple System Atrophy: A Pilot Study

Table 4

ELISA linearity-of-dilution results for the tested RBC sample.

Dilution factor (DF)	Observed (μg/ml) × DF	Expected (μg/ml, neat value)	Recovery (%)
Neat	2.6	2.6	100
1 : 2	3.1		119
1 : 4	3.3		127
1 : 8	3.6		138

Dilutions were made in a previously chosen sample diluent. The observed value was assessed relative to the assay standard curve.