Research Article

Effect of NTN and Lmx1α on the Notch Signaling Pathway during the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells

Figure 1

Gene transfection adopted by recombinant lentivirus to overexpress NTN and Lmx1α. (a) Protein expression was the most abundant when MOI was 50. (b)–(d) Western blot analysis, grayscale analysis, and PCR analysis verified significantly high expression of NTN and Lmx1α in the transfected group relative to the control group. GAPDH was used as an internal control. NTN, neurturin; Lmx1α, LIM homeobox transcription factor 1α; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MOI, multiplicity of infection. . .
(a)
(b)
(c)
(d)