Semaglutide Protects against 6-OHDA Toxicity by Enhancing Autophagy and Inhibiting Oxidative Stress

Figure 2

The effect of semaglutide and liraglutide on autophagy-related protein expression in SH-SY5Y cells with 6-OHDA treatment. (a) LC3-II/LC3-I ratio was downregulated in the 6-OHDA group ( vs. the control group). Semaglutide and liraglutide normalized LC3-II/LC3-I ratio (, vs. 6-OHDA group) and semaglutide was significantly more effective ( vs. 6-OHDA + liraglutide group). (b) Beclin-1 expression was downregulated in the 6-OHDA group ( vs. the control group). Semaglutide and liraglutide normalized beclin-1 expression ( vs. 6-OHDA group) and semaglutide was significantly more effective ( vs. 6-OHDA + liraglutide group). (c) P62 expression was upregulated in the 6-OHDA group ( vs. the control group). Semaglutide and liraglutide normalized P62 expression ( vs. 6-OHDA group) and semaglutide was significantly more effective ( vs. 6-OHDA + liraglutide group). (d) Atg7 expression was downregulated in the 6-OHDA group ( vs. the control group). Semaglutide and liraglutide normalized Atg7 expression ( vs. 6-OHDA group) and semaglutide was significantly more effective ( vs. 6-OHDA + liraglutide group). (e) Western blots of LC3-II, LC3-I, beclin-1, p62, and Atg7 expression. All data were expressed as mean ± standard deviation (n = 4).

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