Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study
Figure 2
RAGE (a)–(c), MnSOD (d)–(f), ECSOD (g)–(i), i-NOS (j)–(l), and nitrotyrosine (m)–(o) expression in UIP. Low power images (a, d, g, j, m) show an overall high degree of protein expression in the lung tissue. In FF, RAGE (b) expression is observed in type II alveolar pneumocytes and stromal cells, MnSOD (e) and ECSOD (h) are expressed in epithelial cells but not in fibroblasts, i-NOS expression (k) is present in inflammatory cells and, to a lower degree, in epithelia; the overall amount of nitrotyrosine is extremely low (n). In bronchiolar basal metaplastic cells of sandwich fibroblast foci, the expression of RAGE (c), MnSOD (f), and ECSOD (i) is less strong than that in overlying alveolar pneumocytes, while i-NOS (l) and nitrotyrosine (o) stain is negative in alveolar pneumocytes, but present in metaplastic cells.
