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Pulmonary Medicine
Volume 2011, Article ID 918036, 10 pages
http://dx.doi.org/10.1155/2011/918036
Research Article

Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung

1The Cardiopulmonary Research Institute and Departments of Medicine and Pediatrics, SUNY Stony Brook School of Medicine, Winthrop University Hospital, Mineola, NY 11507, USA
2Department of Pathology, Thomas Jefferson University Medical Center, Philadelphia, PA 19107, USA
3Departments of Physiology and Pediatrics, Temple University School of Medicine, 3420 North Broad Street, Philadelphia, PA 19140, USA
4Nemours Research Lung Center, Alfred I. DuPont Hospital for Children, Wilmington, DE 19803, USA
5Department of Pediatrics, The Floating Hospital for Children at Tufts Medical Center, Boston, MA 02111, USA
6Center for Inflammation, Translational, and Clinical Lung Research (CILR) and Temple Lung Center, Temple University School of Medicine, Philadelphia, PA 19140, USA

Received 7 March 2011; Accepted 24 May 2011

Academic Editor: Edwin Chilvers

Copyright © 2011 Jeffrey A. Kazzaz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We compared lung delivery methods of recombinant adenovirus (rAd): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2–4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.