Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung

Table 1

Temporal pattern of thoracic luciferase expression.

Vehicle	Viral load (viral particles)	Days after instillation
		1	2	4	7
PP2:PP9	5×10^10	4,021±950	1,127±275	822±100	227±37
Saline	5×10^10	460±665	2,246±2,787	707±675	453±185
PP2:PP9	1×10^10	988±1,237	278±228	313±213	283±299
Saline	1×10^10	232±167	79±63	194±43	260±60

Animals were instilled with either 5×10¹⁰ viral particles (𝑛=3/group) or 1×10¹⁰ viral particles (𝑛=4/group) rAd-Luc intratracheally using either saline or as a PP2:PP9 PFC suspension. Mice were anesthetized, and images were acquired at the time indicated using a cooled CCD camera and imaging system (IVIS50, Xenogen Corp). Luminescence was quantified using Living Image software with a constant region of interest. Values represent the mean ± standard deviation.