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Pulmonary Medicine
Volume 2012, Article ID 746358, 9 pages
http://dx.doi.org/10.1155/2012/746358
Clinical Study

Pulmonary Bacterial Communities in Surgically Resected Noncystic Fibrosis Bronchiectasis Lungs Are Similar to Those in Cystic Fibrosis

1Centre for the Analysis of Genome Evolution & Function, University of Toronto, Toronto, ON, Canada M5S 3B2
2Department of Cell & Systems Biology, University of Toronto, Toronto, ON, Canada M5S 3G5
3Department of Laboratory Medicine & Pathobiology, University of Toronto, Toronto, ON, Canada M5S 1A8
4Latner Thoracic Surgery Research Laboratories, University Health Network, Toronto, ON, Canada
5Division of Respirology, St. Michael’s Hospital and University of Toronto, Toronto, ON, Canada
6Department of Pathology, Toronto General Hospital, University Health Network, Room 11E423, 200 Elizabeth Street, Toronto, ON, Canada M5G 2C4

Received 15 July 2011; Accepted 27 October 2011

Academic Editor: Stefano Centanni

Copyright © 2012 Heather Maughan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Recurrent bacterial infections play a key role in the pathogenesis of bronchiectasis, but conventional microbiologic methods may fail to identify pathogens in many cases. We characterized and compared the pulmonary bacterial communities of cystic fibrosis (CF) and non-CF bronchiectasis patients using a culture-independent molecular approach. Methods. Bacterial 16S rRNA gene libraries were constructed from lung tissue of 10 non-CF bronchiectasis and 21 CF patients, followed by DNA sequencing of isolates from each library. Community characteristics were analyzed and compared between the two groups. Results. A wide range of bacterial diversity was detected in both groups, with between 1 and 21 bacterial taxa found in each patient. Pseudomonas was the most common genus in both groups, comprising 49% of sequences detected and dominating numerically in 13 patients. Although Pseudomonas appeared to be dominant more often in CF patients than in non-CF patients, analysis of entire bacterial communities did not identify significant differences between these two groups. Conclusions. Our data indicate significant diversity in the pulmonary bacterial community of both CF and non-CF bronchiectasis patients and suggest that this community is similar in surgically resected lungs of CF and non-CF bronchiectasis patients.