Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation

Figure 1

PMN quantification in BALF. Rats were pretreated intravenously with buffer (negative or positive controls), CeKI (7.8 or 2.6 mg), or rCeEI (2.6 or 0.84 mg). After 20 min, they received 75 μg LPS/animal (positive control and CeKI and rCeEI groups) or buffer (negative control) injected via the trachea directly into their lungs. Six hours later, lungs were collected and washed with buffer (BALF). BALF (20 µL) was mixed with 0.4% Trypan Blue. Total and differential leukocyte cells were counted manually with a Neubauer chamber using optical microscopy. difference compared to negative control . difference compared to positive control .