Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation

Figure 2

Kinin release in BALF, plasma, and lungs. Rats were pretreated intravenously with buffer (negative or positive controls), CeKI (7.8 or 2.6 mg), or rCeEI (2.6 or 0.84 mg). After 20 min, they received 75 μg of LPS/animal (positive control and CeKI and rCeEI groups) or buffer (negative control) injected via the trachea directly into their lungs. Six hours later, blood was collected, BALF was obtained, and lungs were extracted. Kinin was extracted from BALF (a), plasma (b), or homogenized lung (c) using a treatment with ethanol and then water, acetone, and petroleum ether. For kinin quantification, a radioimmunoassay was performed according to Shimamoto et al., 1978, with some modifications [21]. The experiment was performed twice and radiation values were converted into kinin (pg) using a standard curve. difference compared to negative control (ρ<0.05). difference compared to positive control . difference compared to CeKI-treated groups .

(a)

(b)

(c)