Cardiac PPAR𝛼 Protein Expression is Constant as Alternate Nuclear Receptors and PGC-1 Coordinately Increase During the Postnatal Metabolic Transition

Figure 1

Immunoblots and expression patterns for enzymes controlling substrate oxidation. Data is shown for left ventricle from three development periods (F, N, C) as defined in text. Abbreviations are carnitine palmitoyltransferase I (muscle isoform M-CPTI), carnitine palmitoyltransferase I (liver isoform L-CPTI), hexokinase 2 (HK2), and pyruvate dehydrogenase kinase 2 (PDK2). M-CPTI protein expression decreases occurred between N and C (^*𝑃<.01). L-CPTI protein expression differences decreased immediately after birth F (^*𝑃<.01) and continued to drop in C (⁺𝑃<0.001, versus N). HK2 protein expression exhibited a marked but transient decrease after birth N (^*𝑃<.05, versus F & C). PDK2 protein expression increased with significance noted in C compared to F and N (^*𝑃<.05, F & N).

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