Cryptochrome and Period Proteins Are Regulated by the CLOCK/BMAL1 Gene: Crosstalk between the PPARs/RXR-Regulated and CLOCK/BMAL1-Regulated Systems
Figure 2
Dose-dependent alteration by the CLOCK/BMAL1 gene (0–220 ng) of
the transcriptional activity of PPAR/RXR
mediated through PPRE (-AGGTCA-) elements located in the promoter of acyl-CoA oxidase (AOX) in human kidney 293T
cells in charcoal-treated medium. Alteration by the CLOCK/BMAL1 gene alone (a), plus the PPAR/RXR gene (50 ng) (b), and plus
the PPAR ligand/activator fenofibric acid (10 M) (c) in activity of transcription by PPAR/RXR through PPRE (-AGGTCA-) elements located in the promoter of AOX. Luciferase activity for pAOX-Luc was
normalized to the luciferase activity of pRL-TK. Experiments were performed in
triplicate, and four independent experiments were performed. All data are means
± SD.
