Microglia and Astrocyte Activation by Toll-Like Receptor Ligands: Modulation by PPAR- Agonists
Figure 2
The synthetic PPAR- agonist rosiglitazone attenuates IL-12
p40 production in response to TLR ligands in microglia. Primary microglia
were plated at cells/well in 96-well plates and incubated
overnight. The following day, cells were pretreated for 1 hour with the
indicated concentrations of rosiglitazone prior to the addition of TLR ligands.
Cell-conditioned supernatants were collected at 24 hours following TLR ligand
exposure, whereupon IL-12 p40 levels were determined by ELISA (a). The effects
of rosiglitazone on microglial viability were assessed using an MTT assay (b).
Significant differences between microglia treated with TLR ligands alone versus
TLR ligands + rosiglitazone are noted with asterisks . Results presented are representative of two independent experiments.
