Microglia and Astrocyte Activation by Toll-Like Receptor Ligands: Modulation by PPAR- Agonists
Figure 5
The synthetic PPAR- agonist rosiglitazone selectively
inhibits microglial TNF- expression in response to TLR ligands. Primary microglia were plated at cells/well in 96-well
plates and incubated overnight. The following day, cells were pretreated for 1 hour with the indicated concentrations of rosiglitazone prior to the addition
of TLR ligands. Cell-conditioned supernatants were collected at 24 hours
following TLR ligand exposure, whereupon TNF- levels were determined by ELISA.
Significant differences between microglia treated with TLR ligands alone versus
TLR ligands + rosiglitazone are noted with asterisks . Results presented are representative of two independent experiments.
