PPAR Research / 2008 / Article / Fig 3

Research Article

Improved Insulin Resistance and Lipid Metabolism by Cinnamon Extract through Activation of Peroxisome Proliferator-Activated Receptors

Figure 3

CE increased expression of PPARs and their target genes in differentiated 3T3-L1 cells. 3T3-L1 cells were differentiated in 24-well plate and CE 0.6 mg/mL was added at the same time. On day 5, cells were collected and total RNA was extracted and reversely transcribed into the first strand cDNA with random hexamer primers using cDNA synthesis kit. The gene expression levels were analyzed by quantitative real-time RT-PCR. (a) PPAR and its target genes; (b) PPAR and its target gene; (c) Western blot of CE-treated 3T3-L1 differentiated cells from day 5. 1: Control; 2: DM; 3: Rosiglitazone 1  M; 4: DM + CE 0.2 mg/mL; 5: DM + CE 0.6 mg/mL. For real-time PCR, the results were repeated in at least 3 independent experiments, and -actin mRNA was used as an internal control. Data are presented as mean ± SE. .