Improved Insulin Resistance and Lipid Metabolism by Cinnamon Extract through Activation of Peroxisome Proliferator-Activated Receptors
expression of PPARs and their target genes in differentiated 3T3-L1 cells. 3T3-L1 cells were
differentiated in 24-well plate and CE 0.6 mg/mL was added at the same time. On
day 5, cells were collected and total RNA was extracted and reversely
transcribed into the first strand cDNA with random hexamer primers using cDNA synthesis kit.
The gene expression levels were analyzed by quantitative real-time RT-PCR. (a) PPAR
and its target genes; (b) PPAR and its target gene; (c) Western blot of CE-treated
3T3-L1 differentiated cells from day 5. 1: Control; 2: DM; 3: Rosiglitazone 1 M; 4: DM + CE 0.2 mg/mL; 5: DM + CE 0.6 mg/mL. For real-time PCR, the results
were repeated in at least 3 independent experiments, and -actin mRNA was used
as an internal control. Data are presented as mean ± SE. .