Activation of Penile Proadipogenic Peroxisome Proliferator-Activated Receptor with an Estrogen: Interaction with Estrogen Receptor Alpha during Postnatal Development
Figure 1
(A1) and (A2) RT-PCR amplification of three PPARs (α, β, and γ) from the
body of the penis of three (1, 2, and 3) normal adult (120 days) control rats.
(A1) Shows coamplification of PPARs (α, β, and γ (upper bands) and S15 (small
ribosomal subunit protein as housekeeping gene, lower bands) in two
representative rats (1 and 2). PCR markers were included in lane 1. Expected band sizes for S-15, PPARα, PPARβ, and PPARγ were 361, 492, 390, and 533 bp, respectively. Identities
of amplicons were further confirmed by sequence analysis (see Section 2). Note
that the ampilicons for PPARβ and S15 in
lane 5 and 6 were overlapped (compare run for PPARα, PPARβ, and PPARγ without S15 shown for rat 3 in (A2). In all rats note the
predominant expression of PPARγ. 15 L PCR products were loaded per each lane. (B) Graphic
representation of signal intensity for PPARs showing
predominant expression of PPARγ. Transcript levels were normalized to
the levels of S15 housekeeping gene. To calculate the intensity for PPARβ the mean intensity of S-15 in lanes
2, 3, 8, and 9 in Figure (A1) was subtracted from the combined intensity of PPARβ + S15 in lanes 5 and 6 to obtain the
intensity of penile PPARβ
for rat 1 and 2, respectively. .
