Table of Contents Author Guidelines Submit a Manuscript
PPAR Research
Volume 2008, Article ID 746935, 8 pages
http://dx.doi.org/10.1155/2008/746935
Research Article

Regulation of Peroxisome Proliferator-Activated Receptors by E6-Associated Protein

1The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA
2Center for Molecular Toxicology and Carcinogenesis, Department of Veterinary & Biomedical Sciences, The Pennsylvania State University, University Park, PA 16802, USA

Received 29 May 2008; Revised 25 September 2008; Accepted 5 November 2008

Academic Editor: Wei Xu

Copyright © 2008 Lakshmi Gopinathan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors (NRs) that regulate genes involved in lipid and glucose metabolism. PPAR activity is regulated by interactions with cofactors and of interest are cofactors with ubiquitin ligase activity. The E6-associated protein (E6-AP) is an E3 ubiquitin ligase that affects the activity of other NRs, although its effects on PPARs have not been examined. E6-AP inhibited the ligand-independent transcriptional activity of PPAR and PPAR , with marginal effects on PPAR , and decreased basal mRNA levels of PPAR target genes. Inhibition of PPAR activity required the ubiquitin ligase function of E6-AP, but occurred in a proteasome-independent manner. PPAR interacted with E6-AP, and in mice treated with PPAR agonist clofibrate, mRNA and protein levels of E6-AP were increased in wildtype, but not in PPAR null mice, indicating a PPAR -dependent regulation. These studies suggest coordinate regulation of E6-AP and PPAR , and contribute to our understanding of the role of PPARs in cellular metabolism.