Regulation of Peroxisome Proliferator-Activated Receptors by E6-Associated Protein
Figure 4
E3 ubiquitin ligase
activity of E6-AP is required for regulating PPAR transactivation. (a) 293T cells were transfected with plasmids
expressing 4X-ACO-Luciferase, pRLCMV, PPAR, E6-AP, or E6-AP-C833S that is defective in
ubiquitin ligase function. (b)
Cells were treated with 0.1% DMSO or 5 M MG132 for 6 hours. Cells were lysed and
luciferase activity was corrected for transfection efficiency and protein.
Asterisks indicate a significant difference when compared to the corresponding
0 ratio group. (* with statistical analysis using ANOVA). The
graph is representative of 3 independent experiments.
