Rosiglitazone Inhibits Adrenocortical Cancer Cell Proliferation by Interfering with the IGF-IR Intracellular Signaling
Figure 5
RGZ inhibits IGF-I stimulated PI3K. PI3K activity in SW13 (a) and
H295R (b) treated or not for 15 minutes with 10 nM IGF-I and 20 M RGZ was evaluated by an in vitro assay after immunoprecipitation
with an antibody against the PI3K regulatory subunit p85. The spots correspond
to PI3K catalytic product [32P]phosphatidyl inositol phosphate
(PIP). Representative of two similar experiments. Mean SE (n = 2) of
phospho-Akt band intensity (arbitrary units) is shown in the table under each
blot.
