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PPAR Research
Volume 2009, Article ID 369602, 5 pages
http://dx.doi.org/10.1155/2009/369602
Research Article

Differences in Transcriptional Activation by the Two Allelic (L162V Polymorphic) Variants of PPAR after Omega-3 Fatty Acids Treatment

1Lipid Research Center, CHUL Research Center, QC, Canada G1V 4G2
2Department of Food Science and Nutrition, Laval University, QC, Canada G1V 0A6
3Nutraceuticals and Functional Foods Institute (INAF), Laval University, QC, Canada G1V 0A6
4Laboratory of Molecular Pharmacology, Oncology and Genomic Research Center, CHUL Research Center, QC, Canada G1V 4G2
5Faculty of Pharmacy, Laval University, QC, Canada G1V 0A6

Received 22 August 2008; Revised 26 November 2008; Accepted 21 December 2008

Academic Editor: Mostafa Badr

Copyright © 2009 Iwona Rudkowska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Omega-3 fatty acids (FAs) have the potential to regulate gene expression via the peroxisome proliferator-activated receptor (PPAR ); therefore, genetic variations in this gene may impact its transcriptional activity on target genes. It is hypothesized that the transcriptional activity by wild-type L162-PPAR is enhanced to a greater extent than the mutated variant (V162-PPAR ) in the presence of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) or a mixture of EPA:DHA. To examine the functional difference of the two allelic variants on receptor activity, transient co-transfections were performed in human hepatoma HepG2 cells activated with EPA, DHA and EPA:DHA mixtures. Results indicate that the addition of EPA or DHA demonstrate potential to increase the transcriptional activity by PPAR with respect to basal level in both variants. Yet, the EPA:DHA mixtures enhanced the transcriptional activity to a greater extent than individual FAs indicating possible additive effects of EPA and DHA. Additionally, the V162 allelic form of PPAR demonstrated consistently lower transcriptional activation when incubated with EPA, DHA or EPA:DHA mixtures than, the wild-type variant. In conclusion, both allelic variants of the PPAR L162V are activated by omega-3 FAs; however, the V162 allelic form displays a lower transcriptional activity than the wild-type variant.