A Dominant-Negative PPAR Mutant Promotes Cell Cycle Progression and Cell Growth in Vascular Smooth Muscle Cells
Figure 3
DN-PPAR stimulates DNA synthesis and cell proliferation of quiescent CASMCs. Cells were cultured in serum-depleted medium (0.4% FBS) for 48 hours to induce cell cycle arrest, then infected with Ad-GFP, -DN or -CA (MOI = 40) for 48 hours. (a) Cells were trypsinized, counted, and reseeded into 96-well plates at a density of cells/well. wells/sample. After 3 days culture in serum-depleted medium, incorporation of the BrdU thymidine analog was assayed using a commercially available immunoassay ( versus Ad-GFP control). (b) After infection with virus, cells were reseeded in 12-well plates at a density of cells/well and cultured in serum-depleted medium. After 3 days, cells were harvested and counted on a hemocytometer to measure cell proliferation. counts/sample ( versus Ad-GFP control; versus Ad-GFP control and Ad-DN).
