A Dominant-Negative PPAR Mutant Promotes Cell Cycle Progression and Cell Growth in Vascular Smooth Muscle Cells
Figure 7
DN-PPAR-mediated induction of cell cycle regulators is ERK MAPK-dependent. (a) Ad-DN induced phosphorylation of ERK1/2 and expression of cyclin D1, which are inhibited by the ERK MAPK inhibitor PD98059. (b) PD98059 inhibits DN-PPAR-induced upregulation of phospho-Rb, cyclin A, and MCM7 but increases p27 expression. CASMCs were arrested in serum-deprived medium for 48 hours, cultured with 30 M PD98059 for 30 minutes prior to infection with or without Ad-GFP or -DN recombinant virus for 48 hours, in the absence of serum. Western blot analyses were performed on whole cell extracts of these samples using antibodies specific for the indicated proteins, then stripped and rehybridized with a -actin specific antibody to assess loading variability. Western blots shown are representative of at least three independent experiments.
