The PPAR𝛿 Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells

Figure 11

GW501516 pretreatment has no effect on anisomycin-induced apoptosis. Serum-starved cells were stimulated with (a)–(c) serum-free media (control), (d)–(f) 10⁻⁶ M GW501516 (GW-6), (g)–(i) 10⁻⁸ M GW501516 (GW-8), (j)–(l) 250 ng/mL anisomycin (A250) for 24 hours, (m)–(o) 10⁻⁶ M, or (p)–(r) 10⁻⁸ M GW501516 for 3 hours pretreatment followed by 10⁻⁶ M or 10⁻⁸ M GW501516 for 24 hours with A250 (GW-6/A250 and GW-8/A250). The cells were incubated with terminal transferase and biotin-16-dUTP for one hour, followed by two 45-minute incubations with streptavidin-Cy2 and anti-DAPI. The cells were then mounted on slides, visualized with a fluorescent microscope, and captured with a camera. Apoptotic cells are shown in green, while the blue represents DAPI-positive cells.