Genetic Variability of RXRB, PPARA, and PPARG in Wegener's Granulomatosis
Table 1
Oligonucleotides and restriction
enzymes used for genotyping.
Gene
SNP
Forward (F) and reverse (R) primer ()
Allele specific probes
Restriction enzyme
Fragment lengths
RXRB
rs9277935
F: TGCCCCTTGTAGGTCTCCAC (1)
—
BtgI
T-Allel: 208 bp
T/G
R: CCTCCCACTGTGCCCTAA
G-Allel: 34 + 174 bp
rs2072915
F:
ACATCTCCACCAGCCCCTTC
—
MboI
T-Allel:
325 bp
T/A
R:
GTCCTTCCCCCAGCACAAAG
A-Allel: 224 + 101 bp
rs2744537
F: TCTTCAAGCTCATTGGTGAC
—
BsaI
T-Allel: 451 bp
T/G
R: CCCATTTCCACTCTTCAGAT
G-Allel: 326 + 125 bp
rs1547387
Information not provided by the manufacturer
—
—
C/G
PPARA
rs1800206
Information not provided by the manufacturer
—
—
G/C
rs6008259
F:
CCCCTGTGTCAACAAGATCCA
G:
Fam-CTGTGTTGTCCCCAGCGACCC
—
—
G/A
R:
CTTGAATGGCACAGGGTACATC
A:
YY-CCTGTGTTGTCCCCAACGACCC
PPARG
rs1801282
F:
TTATGGGTGAAACTCTGGGAGATT
G:
Fam-TCCTATTGACGCAGAAAGCGATTCC
—
—
G/C
R:
TTGTGATATGTTTGCAGACAGTGTATC
C:
YY-TCCTATTGACCCAGAAAGCGATTCCTT
rs3856806
F:
CCAGAAAATGACAGACCTCAGACA
T:
Fam-TCACGGAACATGTGCAGCTACTGC
—
—
T/C
R:
GGAGCGGGTGAAGACTCATG
C:
YY-ATTGTCACGGAACACGTGCAGCTAC
(1) A 17 mer nucleotide (GTAAAACGACGGCCAGT) was added 5′ to the forward primer increase fragment length differences between the two alleles,FAM: 6-carboxy-fluorescein; YY: Yakima Yellow.
