Fasting Upregulates PPAR𝛼 Target Genes in Brain and Influences Pituitary Hormone Expression in a PPAR𝛼 Dependent Manner

Figure 3

Effect of fasting on mRNA concentrations of acyl-CoA oxidase (ACO) and mitochondrial 3-hydroxy-3-methylglutaryl-(mHMG)-CoA synthase in pituitary gland and liver of wild-type (+/+) and PPARα knockout mice (−/−). Mice of both genotypes were either fasted for 48 hours (control group) or fed a standard rodent diet ad libitum for 48 hours (fed group). Livers and pituitary glands of mice were excised, pituitary glands from two animals were pooled, total RNA was extracted, and mRNA abundances were determined by real-time detection RT-PCR analysis using 𝛽-actin for normalization. Bars represent means ± SD (liver: 𝑛=10; pituitary: 𝑛=5). Means without a common letter differ, 𝑃<.05. Significant effects (𝑃<.05) from two-way ANOVA: mHMG-CoA synthase (pituitary): genotype, fasting, fasting × genotype (𝑃=.09); ACO (liver): genotype; mHMG-CoA synthase (liver): fasting, genotype, fasting × genotype.

(a)

(b)

(c)

(d)