Fasting Upregulates PPAR Target Genes in Brain and Influences Pituitary Hormone Expression in a PPAR Dependent Manner
Figure 4
Effect of fasting on mRNA concentrations of prolactin, proopiomelanocortin (POMC), luteinizing hormone (LH)-, follicle-stimulating hormone (FSH)-, growth hormone (GH), and thyrotropin (TSH)- in pituitary gland of wild-type () and PPAR knockout mice (). Mice of both genotypes were either fasted for 48ā€‰hours (control group) or fed a standard rodent diet ad libitum for 48ā€‰hours (control group). Pituitary glands of mice were excised, pooled from two animals, total RNA was extracted, and mRNA abundances were determined by real-time detection RT-PCR analysis using -actin for normalization. Bars represent means SD (). Means without a common letter differ, . Significant effects () from two-way ANOVA: prolactin: genotype, fasting genotype; POMC: fasting, genotype; LH: fasting, fasting genotype (); FSH: fasting; TSH: fasting, genotype.