PPAR Research / 2009 / Article / Fig 4

Research Article

Fasting Upregulates PPAR 𝛼 Target Genes in Brain and Influences Pituitary Hormone Expression in a PPAR 𝛼 Dependent Manner

Figure 4

Effect of fasting on mRNA concentrations of prolactin, proopiomelanocortin (POMC), luteinizing hormone (LH)- , follicle-stimulating hormone (FSH)- , growth hormone (GH), and thyrotropin (TSH)- in pituitary gland of wild-type ( ) and PPAR knockout mice ( ). Mice of both genotypes were either fasted for 48 hours (control group) or fed a standard rodent diet ad libitum for 48 hours (control group). Pituitary glands of mice were excised, pooled from two animals, total RNA was extracted, and mRNA abundances were determined by real-time detection RT-PCR analysis using -actin for normalization. Bars represent means SD ( ). Means without a common letter differ, . Significant effects ( ) from two-way ANOVA: prolactin: genotype, fasting genotype; POMC: fasting, genotype; LH : fasting, fasting genotype ( ); FSH : fasting; TSH : fasting, genotype.

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