Research Article
Fasting Upregulates PPAR Target Genes in Brain and Influences Pituitary Hormone Expression in a PPAR Dependent Manner
Table 1
Characteristics of the specific primers used for RT-PCR analysis.
| | Gene | Forward primer (5’ to 3’) | Reverse primer (5’ to 3’) | PCR product size (bp) | NCBI GenBank |
| | β-Actin (rat) | ATCGTGCGTGACATTAAAGAGAAG | GGACAGTGAGGCCAGGATAGAG | 429 | BC063166 | | ACO (rat) | CTTTCTTGCTTGCCTTCCTTCTCC | GCCGTTTCACCGCCTCGTA | 415 | NM017340 | | CPT1A (rat) | GGAGACAGACACCATCCAACATA | AGGTGATGGACTTGTCAAACC | 416 | NM031559 | | CPT1C (rat) | CATCTCCAGCAAGCAATCAA | GATCCCCAATACCCCTGTCT | 299 | BC105882 | | LCAD (rat) | AAGGATTTATTAAGGGCAAGAAGC | GGAAGCGGAGGCGGAGTC | 380 | NM012849 | | MCAD (rat) | CAAGAGAGCCTGGGAACTTG | CCCCAAAGAATTTGCTTCAA | 154 | NM016986 | | Mitochondrial HMG-CoA synthase (rat) | GGCCTTGGACCGATGCTATGC | GGGAGGCCTTGGTTTTCTTGTTG | 323 | BC083543 | | PPAR (rat) | CCCTCTCTCCAGCTTCCAGCCC | CCACAAGCGTCTTCTCAGCCATG | 555 | NM013196 | | β-Actin (mouse) | ACGGCCAGGTCATCACTATTG | CACAGGATTCCATACCCAAGAAG | 87 | NM007393 | | ACO (mouse) | CAGGAAGAGCAAGGAAGTGG | CCTTTCTGGCTGATCCCATA | 189 | NM015729 | | LHβ (mouse) | GTCCCAGGACTCAACCAATG | GGGAGGGAGGGATGATTAGA | 110 | NM008497 | | Mitochondrial HMG-CoA synthase (mouse) | CCTCTGTGAATCCTGGGTGT | CTGTGGGGAAAGATCTGCAT | 141 | NM008256 | | POMC (mouse) | GGGTCCCTCCAATCTTGTTT | GCACCAGCTCCACACATCTA | 137 | NM008895 | | Prolactin (mouse) | CTCAGGCCATCTTGGAGAAG | TCGGAGAGAAGTCTGGCAGT | 174 | NM011164 | | FSHβ (mouse) | AGGGAGGAAAGGAAAGTGGA | AGCCAGCTTCATCAGCATTT | 202 | NM008045 | | GH (mouse) | ACGCGCTGCTCAAAAACTAT | GCTAGAAGGCACAGCTGCTT | 120 | NM008117 | | TSHβ (mouse) | TCAACACCACCATCTGTGCT | TCTGACAGCCTCGTGTATGC | 239 | NM009432 |
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