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PPAR Research
Volume 2009, Article ID 874126, 5 pages
http://dx.doi.org/10.1155/2009/874126
Research Article

Impact of the Pro12Ala Polymorphism of the PPAR-Gamma 2 Gene on Metabolic and Clinical Characteristics in the Palestinian Type 2 Diabetic Patients

1Department of Biochemistry and Molecular Biology, Medical School, Al-Quds University, East Jerusalem, Abu-Deis, P.O. Box 19356, Palestine
2Al-Quds Nutrition and Health Research Institute, Al-Quds University, East Jerusalem, P.O. Box 20760, Palestine

Received 14 June 2009; Revised 6 August 2009; Accepted 25 August 2009

Academic Editor: Mostafa Badr

Copyright © 2009 S. Ereqat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Peroxisome proliferators activated receptor-gamma2 (PPARγ2) represents the transcriptional master regulator of adipocyte differentiation and therefore has been suggested as a candidate gene for obesity, insulin resistance, and dyslipidemia. The objective of the study was to investigate for the first time the potential association of the most common variant Pro12Ala (p.P12A) substitution of the PPARγ2 gene with body mass index (BMI), blood pressure, fasting plasma glucose, plasma total cholesterol, LDL and HDL cholesterol, and plasma triglyceride in a sample of 202 (138 females and 64 male) type 2 diabetic Palestinians. Genotyping of the PPARγ2 p.P12A polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The A12 allele was associated with lower fasting plasma glucose (P=.03) but had no influence on blood pressure, BMI, or other metabolic parameters. In obese patients, the p.P12A substitution was associated with elevated total plasma cholesterol levels (P=.02) and a tendency toward increased LDL cholesterol level (P=.06). In conclusion, the p.P12A variant of the PPARγ2 may influence cardiovascular risk through effects on lipid metabolism in obese T2D Palestinian patients.