WY-14643, a Potent Peroxisome Proliferator Activator Receptor-α PPAR-α Agonist Ameliorates the Inflammatory Process Associated to Experimental Periodontitis
Figure 3
Effect of WY14643 on cytokines expression. Immunohistochemical analysis of gingivomucosal tissues from the contra lateral side obtained from vehicle-treated rats did not reveal any immunoreactivity for TNF-α and IL-1β (a, e). Positive staining for for TNF-α and IL-1β were found in the gingivomucosal tissues from ligature operated rats (b, f) after ligature. WY14643 treatment significantly reduced the degree of positive staining for TNF-α and IL-1β (c, g). Densitometry analysis of immunocytochemistry photographs (d, h); photos from each samples collected from all rats in each experimental group) for TNF-α and IL-1β from gingivomucosal tissue was assessed. The assay was carried out by using Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Densitometry data are expressed as % of total tissue area.
