WY-14643, a Potent Peroxisome Proliferator Activator Receptor-α PPAR-α Agonist Ameliorates the Inflammatory Process Associated to Experimental Periodontitis
Figure 4
Effects of WY14643 on the expression of ICAM-1 and neutrophils infiltration. Immunohistochemical analysis of gingivomucosal tissues from the contra lateral side obtained from vehicle-treated rats did not reveal any immunoreactivity for ICAM-1. (a) Positive staining for ICAM-1 was found in the gingivomucosal tissues from ligature operated rats. (b) In contrast, a positive staining for ICAM-1 was significantly attenuated by the treatment with WY14643. (c) Moreover myeloperoxidase activity (e) in gingivomucosal tissue was significantly increased by ligature compared to the contra lateral side obtained from vehicle-treated rats. WY14643 significantly reduced myeloperoxidase activity levels. Figure is representative of at least 3 experiments performed on different experimental days. Densitometry analysis of immunocytochemistry photographs; (d) photos from each samples collected from all rats in each experimental group) for ICAM-1 from gingivomucosal tissue was assessed. The assay was carried out by using Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Densitometry data are expressed as % of total tissue area. Data are means of mean ± s.e.m. from rats for each group. versus nonligated. versus ligated.
