Involvement of Fatty Acid Binding Protein 5 and PPAR/ in Prostate Cancer Cell Growth
Figure 4
The FABP5/PPAR pathway enhances prostate cancer cell proliferation and transformation. (a) PC3M cells that stably express shFABP5 were cultured in a 24 well plate (2500 cells/well) and treated with vehicle or GW 0742 (1 M). Cells were counted at the denoted days. Data are meanSEM (). Inset: immunoblotting demonstrating low FABP5 level in shFABP5-expressing. (b) PC3M cells were transfected with a vector harboring PPARsiRNA. Four days later, were cultured in a 24 well plate (2500 cells/well) and treated with vehicle or GW 0742 (1 M). Cells were counted at the denoted days. Inset: Q-PCR analyses demonstrating low PPAR in cells transfected with SiRNA towards the receptor. (c) Colony formation assays were conducted in the absence or presence of GW0742 (1 M). Cells were cultured in 2% agarose for 29 days (see Materials and methods for details). Media was replenished every 4 days. Colonies were visualized by staining with 0.005% crystal violet and counted under a light microscope. Data are meanSEM ().
