Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPAR𝛼

Table 3

Validation of gene expression results from microarray analysis using qRT-PCR.

Gene symbol	Fold change microarray		Fold change qRT-PCR Canx
	Il10^−/− versus C57 7 weeks	Il10^−/− versus C57 12 weeks	Il10^−/− versus C57 7 weeks	Il10^−/− versus C57 12 weeks
Abcb1A	−3.4	−2.8	−5.2	−4.9
Aldh1A1	−1.5^∗	−3.0	−1.5^#	−3.4
Ces2	−1.8^∗	−3.1	−2.9	−5.6
Fabp2	−1.9	−5.6	−1.5^#	−9.8
Igfbp5	−1.5^∗	1.6^∗	−1.9^#	2.8
Il1B	1.8^∗	6.4	3.4	8.5
Mmp13	1.0^∗	1.8	2.7^#	9.9
Ppara	−2.0	−1.7	−5.6	−3.8
Srebf1	1.0^∗	1.0^∗	1.2^#	1.8
Sult1A1	−3.2	−3.1	−3.6	−3.3

Canx (calnexin) reference gene used to to normalize the data; *microarray result was not significantly different using moderated 𝑡-statistics and false discovery rate (FDR) control in limma; genes that satisfied the criterion of FC ≥ 1.5 and 𝑞<0.05 were considered to be significantly different; ^#qRT-PCR result was not significantly different using ANOVA.