Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPAR𝛼

Table 4

Proteins differentially expressed in the colon of Il10^−/− mice compared to C57 mice at 7 and 12 weeks of age.

Spot	Accession number	Protein description	Gene (MGI)	Theoretical Mr (kDa)	Theoretical pI	No. peptides matched	Sequence coverage %	SEQUEST P (pro)	Fold change
7 weeks
1	gi∣157951604	CAP, adenylate cyclase-associated protein 1	Cap1	51.5	7.29	2	4.2	9.50E-05	−2.9
2	gi∣6680027	glutamate dehydrogenase 1	Glud1	61.3	7.96	3	6.1	2.85E-10	−2.9
3	gi∣6671549	peroxiredoxin 6	Prdx6	24.8	5.96	5	27.2	3.99E-08	3.4
12 weeks
4	gi∣6752954	actin, gamma, cytoplasmic 1	Actg1	41.8	5.20	10	38.9	7.54E-12	−3.2
5	gi∣6671507	actin, alpha 2, smooth muscle, aorta	Acta2	42.0	5.12	3	13.3	2.13E-06	−3.2
6	gi∣6752954	actin, gamma, cytoplasmic 1	Actg1	41.8	5.20	9	30.7	2.76E-10	−7.4
7	gi∣148666671	actin, gamma 2, smooth muscle, enteric	Actg2	41.9	5.20	4	20.7	1.50E-06	−7.4
8	gi∣6752954	actin, gamma, cytoplasmic 1	Actg1	41.8	5.20	6	15.7	7.93E-07	−2.9
9	gi∣21312260	aldehyde dehydrogenase 1 family, member B1	Aldh1b1	57.5	6.61	8	16.6	2.88E-09	−3.3
10	gi∣160333304	apolipoprotein A-I	Apoa1	30.6	5.57	4	16.7	5.56E-12	2.4
11	gi∣33563236	Rho, GDP dissociation inhibitor (GDI) beta	Arhgdib	22.8	4.80	4	22.5	4.11E-13	2.9
12	gi∣6680748	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1	Atp5a1	59.7	9.53	15	35.1	3.40E-12	−2.0
13	gi∣6680748	ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1	Atp5a1	59.7	9.53	12	27.8	1.72E-12	−2.1
14	gi∣31980648	ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit	Atp5b	56.3	5.07	16	41.8	5.47E-12	−2.1
15	gi∣6753244	calmodulin 1	Calm1	16.8	3.93	3	32.9	6.21E-11	5.0
16	gi∣145301561	carbonic anhydrase 1	Car1	28.3	6.49	7	36.4	4.32E-10	2.3
17	gi∣126521835	chaperonin subunit 2 (beta)	Cct2	57.4	5.96	11	26.7	2.76E-09	−2.2
18	gi∣6680924	cofilin 1, non-muscle	Cfl1	18.5	8.20	4	18.8	8.89E-09	2.9
19	gi∣6680924	cofilin 1, non-muscle	Cfl1	18.5	8.20	4	18.8	1.28E-09	2.5
20	gi∣6755522	evolutionarily conserved signalling intermediate in Toll pathway	Ecsit	49.7	6.14	2	4.1	1.54E-05	−2.3
21	gi∣33859482	eukaryotic translation elongation factor 2	Eef2	95.3	6.30	10	13.4	8.98E-12	−5.8
22	gi∣123261836	EF hand domain containing 2	Efhd2	26.8	4.92	5	24.2	3.42E-08	2.0
23	gi∣31712036	eukaryotic translation initiation factor 5A	Eif5a	16.8	4.94	3	20.8	9.81E-12	2.2
24	gi∣14149635	fatty acid binding protein 4, adipocyte	Fabp4	14.6	8.74	5	48.5	1.28E-08	2.9
25	gi∣6680027	glutamate dehydrogenase 1	Glud1	61.3	7.96	10	20.1	7.64E-11	−2.9
26	gi∣123123547	gelsolin	Gsn	85.9	5.78	5	10.4	5.61E-07	−2.3
27	gi∣123123547	gelsolin	Gsn	85.9	5.78	3	4.4	2.19E-07	−2.5
28	gi∣56238582	histidine triad nucleotide binding protein	Hint1	13.8	6.41	2	16.7	1.39E-10	2.1
29	gi∣62024583	Cr1 protein	Igkv1-117	26.3	7.97	2	11.3	2.05E-09	2.2
30	gi∣114145561	keratin complex 2, basic, gene 8	Krt8	54.5	5.59	16	39.6	1.20E-11	−2.0
31	gi∣123236748	keratin complex 1, acidic, gene 19	Krt19	44.5	5.15	6	12.9	6.39E-10	−3.2
32	gi∣123236748	keratin complex 1, acidic, gene 19	Krt19	44.5	5.15	13	45.4	1.82E-08	−7.4
33	gi∣123236748	keratin complex 1, acidic, gene 19	Krt19	44.5	5.15	9	25.3	8.96E-10	−2.9
34	gi∣40556608	heat shock protein 1, beta	Hsp90ab1	83.2	4.82	15	22.1	2.47E-10	−2.8
35	gi∣40556608	heat shock protein 1, beta	Hsp90ab1	83.2	4.82	17	25.3	1.01E-11	−2.1
36	gi∣26984237	mitochondrial ATP-dependent protease Lon	Lonp1	10.6	6.20	4	4.0	5.94E-05	−3.0
37	gi∣149266035	PREDICTED: similar to non-muscle myosin alkali light chain	Myl6	13.0	4.42	3	34.5	7.28E-06	5.1
38	gi∣6755040	profilin 1	Pfn1	15.0	8.43	6	55.7	1.42E-12	2.9
39	gi∣51772387	PREDICTED: similar to 40S ribosomal protein S12	Rsp12	14.5	6.94	2	12.1	4.18E-04	2.1
40	gi∣33859624	S100 calcium binding protein A4	S100a4	11.7	5.07	2	17.8	1.43E-04	2.4
41	gi∣6677837	S100 calcium binding protein A9 (calgranulin B)	S100a9	13.0	6.76	4	36.3	7.54E-06	4.0
42	gi∣18314646	S100 calcium binding protein A11 (calgizzarin)	S100a11	11.1	5.15	1	16.3	1.65E-04	5.1
43	gi∣18314646	S100 calcium binding protein A11 (calgizzarin)	S100a11	11.1	5.15	1	16.3	3.53E-05	3.2
44	gi∣14389431	stress-induced phosphoprotein 1	Stip1	62.5	6.39	7	11.6	1.31E-05	−3.9
45	gi∣27754031	sorting nexin 6	Sx6	45.0	5.47	3	7.14	2.62E-05	−2.3
46	gi∣226958349	triosephosphate isomerase 1	Tpi1	26.7	7.07	3	19.3	6.59E-09	2.1
47	gi∣6678469	tubulin, alpha 1C	Tuba1c	50.1	4.83	12	34.5	1.26E-11	−2.0
48	gi∣7106439	tubulin, beta 5	Tubb5	49.7	4.64	9	34.7	1.30E-11	−2.2
49	gi∣123210316	thioredoxin	Txn	11.7	4.64	4	31.4	4.27E-07	2.5
50	gi∣148877507	villin 1	Vil1	92.7	5.66	13	18.7	8.57E-09	−3.2
51	gi∣148877507	villin 1	Vil1	92.7	5.66	20	26.4	4.52E-08	−4.6
52	gi∣83921618	villin 2	Vil2	69.4	5.76	7	14.8	3.15E-09	−2.4
53	gi∣83921618	villin 2	Vil2	69.4	5.76	8	15.9	1.99E-08	−2.6