Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Figure 1
Effects of selective PPAR agonists on cell proliferation in rat chondrocytes stimulated with TGF-1. Chondrocytes, embedded in alginate beads (5.104 cells per bead), were exposed to Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10 M) for 2 h before stimulation with TGF-1 (10 ng/mL), in the presence or absence of PPAR agonists, for 72 h. Levels of total DNA were measured by Hoechst assay. (a) Time course effect of TGF-1; (b) effect of PPAR agonists in presence of TGF-1. Data are expressed as mean ± SEM.
