Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Figure 5
Selective reversion of potency of PPAR agonists on collagens’ deposition in rat chondrocytes. Chondrocytes, embedded in alginate beads (5.104 cells per bead), were pretreated for 2 h with Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10 M), in the presence or absence of synthetic PPAR (GW6471, 10 M) or synthetic PPAR (GW9662, 10 M) antagonists, before stimulation with 10 ng/mL TGF-1 for 72 h. (a) Collagens’ deposition in the extracellular matrix (cell-associated matrix) after coloration of representative sections of beads (, G: 400X) by Sirius red after 72 h. (b) Measurement of Sirius red staining of the representative section of beads using NIH/Image software (). Data were expressed as mean ± SEM and statistically significant differences from the control were indicated as ; from TGF-1-treated cells as ; from “TGF-1+agonists”-treated cells as .
