Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Figure 6
Selective reversion of potency of PPAR agonists on type II collagen mRNA expression in rat chondrocytes. Chondrocytes, embedded in alginate beads (5.104 cells per bead), were pretreated for 2 h with Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10 M), before stimulation with 10 ng/mL TGF-1 for 48 h. Normalized collagen type II mRNA levels were quantified by real-time PCR (). Data were expressed as mean ± SEM and statistically significant differences from the control were indicated as ; from TGF-1-treated cells as ; from “TGF-1+agonists”-treated cells as
