Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Figure 7
Selective reversion of potency of PPAR agonists on global MMP (-1, -2, -3, -7, and -9) activity in rat chondrocytes. Chondrocytes, embedded in alginate beads (5.104 cells per bead), were pretreated for 2 h with Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10 M), in the presence or absence of synthetic PPAR (GW6471, 10 M) or synthetic PPAR (GW9662, 10 M) antagonists, before stimulation with 10 ng/mL TGF-1 for 72 h. Global MMP (-1, -2, -3, -7, and -9) activities was evaluated by fluorigenic assays in culture supernatants (). Data were expressed as mean ± SEM and statistically significant differences from the control were indicated as , from TGF-1-treated cells as , and from “TGF-1+agonists”-treated cells as .
