Research Article
Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Table 2
Effects of selective PPAR agonists on TGF-1-induced changes in MMPs and TIMP-1 genes expression in rat chondrocytes. Chondrocytes, embedded in alginate beads ( cells per bead), were exposed to Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10 M) for 2 h before stimulation with TGF-1 (10 ng/mL), in the presence or absence of PPAR agonists, for 48 h. Normalized mRNA levels of MMP-3, MMP-13, and TIMP-1 quantified by real-time PCR (). Data are expressed as mean ± SEM. Statistically significant differences from the control are indicated as , and from TGF-1-treated cells as .
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