Coordinate Transcriptomic and Metabolomic Effects of the Insulin Sensitizer Rosiglitazone on Fundamental Metabolic Pathways in Liver, Soleus Muscle, and Adipose Tissue in Diabetic db/db Mice
Figure 4
Expression profiles of key genes involved in IAT FA metabolism. Differentially expressed genes in db/+ (the white square) as well as 10 (the off-white square), 30 (the grey square), and 100 (the black square) mg/kg RSG-treated db/db mice were measured versus untreated db/db and plotted as the mean (% increase/decrease of db/db control) SEM (). NS; otherwise (a) Genes related to FA transport, glucose, FA and TG synthesis and pentose pathway*: Agpat3, 1-acylglycerol-3-phosphate O-acyltransferase 3; Dgat1, diacylglycerol O-acyltransferase 1; Slc27a1, solute carrier family 27 (fatty acid transporter), member 1; Slc2a4, solute carrier family 2 (facilitated glucose transporter), member 4. (b) Genes related to mitochondrial and peroxisomal -oxidation: Acaa1a, Acaa1b, acetyl-Coenzyme A acyltransferase 1A, 1B; Acaa2, acetyl-Coenzyme A acyltransferase 2; Acadl, acyl-Coenzyme A dehydrogenase, long chain; Acadm, acyl-Coenzyme A dehydrogenase, medium chain; Acads, acyl-Coenzyme A dehydrogenase, short chain; Acadvl, acyl-Coenzyme A dehydrogenase, very long chain; Acox1, acyl-Coenzyme A oxidase 1, palmitoyl; Cpt2, carnitine palmitoyltransferase 2; Crat, carnitine acetyltransferase; Dci, dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase); Echs1, enoyl-Coenzyme A hydratase, short chain 1 mitochondrial; Ehhadh, enoyl-Coenzyme A, hydratase/3-hydroxyacyl-Coenzyme A dehydrogenase; Hadhb, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase, beta subunit. (c) Genes related to citrate metabolism and oxidative phosphorylation: Atp5a1, ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit, isoform 1; Atp5b, ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; Cox1, M. musculus mRNA for mitochondrial gene for subunit I of cytochrome c oxidase; Cs, citrate synthase; Fh1, fumarate hydratase 1; Mdh2, malate dehydrogenase 2, NAD (mitochondrial); Sdha, succinate dehydrogenase complex, subunit A, flavoprotein (Fp); Sdhb, succinate dehydrogenase complex, subunit B, iron sulfur (Ip); Sucla2, succinate-Coenzyme A ligase, ADP-forming, beta subunit; Uqcrc1, Uqcrc 2, ubiquinol-cytochrome c reductase core protein 1, 2; Uqcrfs1, ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1; Uqcrq, ubiquinol-cytochrome c reductase, complex III subunit VII. *gene abbreviations given in Figure 1 were not mentioned.
