Regulation of ENaC-Mediated Sodium Reabsorption by Peroxisome Proliferator-Activated Receptors

Figure 2

PPAR$\alpha$ inhibits ENaC when overexpressed in CHO cells. (a) Overlays of typical macroscopic current traces before (arrow) and after $10\hspace{0.17em}\mu \text{M}$ amiloride from voltage-clamped CHO cells transfected with mENaC alone (top) and with PPAR$\alpha$. Currents were elicited by voltage ramping from 60 mV down to $-100\hspace{0.17em}\text{mV}$ (holding potential is $40\hspace{0.17em}\text{mV}$). (b) Summary graph of ENaC activity in CHO cells when all three mENaC subunits are expressed in the absence and presence of PPAR$\alpha$. The numbers of experiments are 9 and 7 for control and cells overexpressed with PPAR$\alpha$ cDNA ($1\hspace{0.17em}\mu \text{g}$), respectively.

(a)

(b)