Combination PPAR𝛾 and RXR Agonist Treatment in Melanoma Cells: Functional Importance of S100A2

Figure 2

Protein expression of S100A2 in A375(DRO) cells. 60 μg of nuclear protein extract from A375(DRO) before (DMSO—lane 1) and after combination treatment (lane 2) or transfected with empty vector (EV—lane 3) or S100A2 in pcDNA3 vector with no treatment (lane 4) was size-separated on a 10% SDS-PAGE gel and transferred to nitrocellulose. The blot was blocked with 10% nonfat milk and incubated with S100A2 receptor antibodies (sc Y-20). β-Actin was measured as a loading control.