Combination PPAR𝛾 and RXR Agonist Treatment in Melanoma Cells: Functional Importance of S100A2

Figure 6

Western blot of rexinoid receptors in A375(DRO) cells after knock down of S100A2. 60 μg of nuclear protein extract from A375(DRO), the SCR shRNA-infected control cell, and a clone of shS100A2 infected cells were size-separated on a 10% SDS-PAGE gel and transferred to nitrocellulose. The blot was blocked with 10% nonfat milk and incubated with PPAR$\gamma$, RXRγ, and RXRα primary antibodies and then secondary antibody with antirabbit IgG conjugated to horse-radish peroxidase as previously described. PARP was measured as a loading control.