PPAR-𝛼 Contributes to the Anti-Inflammatory Activity of Verbascoside in a Model of Inflammatory Bowel Disease in Mice

Figure 6

Effects of PPAR-$\alpha$ on intestinal permeability. Permeability probes (lactulose and mannitol) were administered by gavage, and urinary lactulose and mannitol excretion were measured in urine collected over a 4- to 5-hour postgavage period, and L/M ratios were calculated. The L/M ratio was unchanged in the sham-treated mice. On the contrary, at 4 days after colitis, the L/M ratios were significantly higher in the urinary samples from DNBS-treated PPAR-$\alpha$WT mice in comparison with the sham animals. The absence of a functional PPAR-$\alpha$ gene in PPAR-$\alpha$KO mice resulted in a more pronounced increase of L/M ratios. Treatment with VB significantly reduced the increased L/M ratios, while the absence of PPAR-$\alpha$ gene significantly abolished the effect of VB. Data are means $\pm$ SEM of 10 mice for each group. *$P\mathrm{\hspace{0.17em}\hspace{0.17em}}<\mathrm{\hspace{0.17em}\hspace{0.17em}}.01$ versus sham; °$P\mathrm{\hspace{0.17em}\hspace{0.17em}}<\mathrm{\hspace{0.17em}\hspace{0.17em}}.01$ DNBS WT.