Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet

Figure 2

Progressive infiltration of pro-inflammatory ATMs assessed by flow cytometry. The epididymal fat pads were isolated from $n\ge 7$ animals per group at each time point. The SVF was isolated and stained to identify total leukocytes (b), macrophages (c), the pro-inflammatory (d) and resident (e) ATM subsets according to the gating strategy (a). The ratio of the pro-inflammatory ATMs over the resident ATMs is a quick indicator of the phenotypic profile of the SVF macrophages. Values represent the mean and SEM. The Chow group is represented by circles and dashed lines and the DIO group on HF/CH diet is represented by squares and a solid line. Unpaired T test was used to compare standard chow to DIO at each time point; *$P\le .05$, **$P\le .01$, and ***$P\le .001$.