Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet

Figure 7

Rosiglitazone can modulate a trend for an elevation in the percentage of monocytes. Whole blood was stained and monocytes were assessed in the kinetic timecourse at 3, 6, 16, and 27-weeks, as well as in the Rosiglitazone study terminated at 18 weeks. (a) and (b) show the gating strategy and a representative dot plot that were employed to identify the monocyte populations. (c)-(d). In the kinetic study, results show a trend for an increase in the percentage of both Ly6Chi and Ly6Clo monocyte populations in the DIO cohort compared to chow, but not in absolute numbers. (e)-(f). Rosiglitazone nonsignificantly reduced the elevation in both the Ly6C high and Ly6Clo monocyte percentages and the Ly6Chi but not Ly6Clo absolute numbers.