Rosiglitazone Induces Mitochondrial Biogenesis in Differentiated Murine 3T3-L1 and C3H/10T1/2 Adipocytes
Figure 5
RSG increased O2 consumption rate in 3T3-L1 adipocytes. (a) After treatment with RSG (1 μM) or DMSO ( each) for 48 hrs, 3T3-L1 adipocytes were transferred to the Oxygen Biosensor plates, and assayed for O2 consumption at 0, 2, 4, 6, 8, 10, and 12 hrs after the transfer (see Methods for more details). * versus DMSO at matching time points. (b) After treatment with RSG (10, 3.3, 1.1, 0.37, 0.12, 0.041, 0.014, 0.0046, 0.0015, and 0.00051 μM) or DMSO ( each condition) for 48 hrs, 3T3-L1 adipocytes were transferred to the Oxygen Biosensor plates and assayed for O2 consumption 6 hrs after the transfer. All numerical data were expressed as mean ± S.E. All experiments were repeated at least once.
