Research Article
Consequences of PPARα Invalidation on Glutathione Synthesis: Interactions with Dietary Fatty Acids
Table 1
Primer sequences used in quantitative RT-PCR analysis.
| | Gene name | Abbreviation | Ref Seq | Forward primer | Reverse primer |
| | Glutathione peroxidase 1 (GPX1) | Gpx1 | NM_008160 | GACACCAGGAGAATGGCAAGA | ACCATTCACTTCGCACTTCTCA | | Cysteine dioxygenase (CDO) | Cdo | NM_033037 | GATACATGCCACGCCTTTGA | CCTGAAGTTGTAAATGGAGTCCTGAT | | Catalase (CAT) | Cat | NM_009804 | GCCAGAAGAGAAACCCACAGACT | CACTGAACAAGAAAGAAACCTGATG | | Glutamate cysteine ligase (γGCL), catalytic subunit | Gclc | NM_010295 | GGAGGCGATGTTCTTGAGACTCT | CCTTCGATCATGTAACTCCCATACT | | Glutamate-cysteine ligase (γGCL), modifier subunit | Gclm | NM_008129 | GGCCTCCTGCTGTGTGATG | GCCTCAGAGAGCAGTTCTTTCG | | Superoxide dismutase 1 (SOD1) | Sod1 | NM_011434.1 | GTGCAGGGAACCATCCACTT | GTCCTGACAACACAACTGGTTCA | | Superoxide dismutase 2 (SOD2) | Sod2 | NM_013671 | GCTCTGGCCAAGGGAGATG | TGATTAATATGTCCCCCACCATT | | CD68 antigen (CD68) | Cd68 | NM_009853.1 | CATCAGAGCCCGAGTACAGTCTACC | AATTCTGCGCCATGAATGTCC | | Chemokine (C-C motif) ligand 2 (MCP1) | Ccl2 | NM_011333.3 | GGCTCAGCCAGATGCAGTTAA | CCAGCCTACTCATTGGGATCA | | Serum amyloid A (SAA) | Saa | NM_009117.3 | GCGAGCCTACACTGACATGA | TTTTCTCAGCAGCCCAGACT |
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All primer sequences were designed using Primer Express (Applied Biosystems) software and were from Eurogentec (Eurogentec, Seraing, Belgium).
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