Downregulation of the Proinflammatory State of Circulating Mononuclear Cells by Short-Term Treatment with Pioglitazone in Patients with Type 2 Diabetes Mellitus and Coronary Artery Disease

Table 1

Primer composition and PCR protocols.

Gene	Forward primer; Reverse primer	Annealing temperature [°C]	Annealing time [s]	Elongation time [s]	Product melting temperature [°C]
Rel-A	CAGTACCTGCCAGATACAGACGA; GGGAAGGCACAGCAATG	63	10	7	88
P105	TGATGATTTACTAGCACAAGGAGACAT; TGTACCCCCAGAGACCTCATAG	65	10	9	85
IκB-α	CTGATGTCAATGCTCAGGAGCC; TGTGTCATAGCTCTCCTCATCCTCAC	68	10	11	89
IκB-ß	CTGAAAACTACGAGGGCCA; CCTCCACTGCCAAATGAAG	64	5	8	91
TNF-α	CCCAGGGACCTCTCTCTAATC; ATGGGCTACAGGCTTGTCACT	64	10	8	87
IL-6	CCCATGCAGGCACTTACTAC; ACGTCTTCTTGAACCTCAGAACA	63	5	4	86
MIF	CGGACAGGGTCTACATCAA; CTTAGGCGAAGGTGGAGTT	63	5	4	84
MMP-9	CCCATTTCGACGATGACGAGTTGTG; GGAGTAGGATTGGCCTTGGAAGATG	64	10	13	92