753917.fig.003a
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753917.fig.003b
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753917.fig.003c
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Figure 3: PPAR mediated alteration in Egr-1/MCP-1 protein levels by ω-6 lipids. Egr-1 and MCP-1 protein levels were determined using Western blot in cells exposed to ω-6 lipids after pretreatment with PPARα or γ antagonists, MK886 or GW9662. Control-(CTRL) vehicle only. The results were expressed as mean ± SEM (Standard Error of Mean). (a–c) Egr-1 protein levels after 1, 4, and 12 hrs; (d–f) MCP-1 protein levels after 1, 4, and 12 hrs. One way ANOVA was used for the comparison between two treatments. Significance was confirmed using post hoc Fisher LSD test. * 𝑃 < 0 . 0 5 .